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SOLVED: Below are the key features of vector B and the steps for cloning the gene into the vector f in order to create the plasmid A, which is then transformed into JMI09 E. Coli. In principle, JMI09 E. Coli transformed with plasmid A will be able to metabolize X-gal. Key features of vector B: - Multiple cloning site that is compatible with HindIII, AluI, EcoRI, BamHI, XbaI - A kanamycin resistance gene - A replicon - 9916 bp in size Steps for cloning and transformation:
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