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SOLVED: The hydrolysis of pyrophosphate to orthophosphate is important in driving forward biosynthetic reactions such as the synthesis of DNA. This hydrolytic reaction is catalyzed in E. coli by pyrophosphatase, which has a mass of 120 kDa and consists of six identical subunits. For this enzyme, the unit of activity is defined as the amount of enzyme that hydrolyzes 10 mmol of pyrophosphate in 15 minutes at 37°C under standard assay conditions. The purified enzyme has a Vmax of 2800 units per m
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