Scientific Research Publishing

Springer, N.M. (2010) Isolation of Plant DNA for PCR and Genotyping Using Organic Extraction and CTAB. Cold Spring Harbor Protocols, 2010, pdb.prot5515.

ABSTRACT: Polymerase Chain Reaction (PCR) amplification of genomic DNA is a crucial step for molecular studies such as sequencing and genotyping because it requires pure and high-quality DNA. However, ...
Scientific Research Publishing

Huang, X., Duan, N., Xu, H., Xie, T.N., Xue, Y.-R. and Liu, C.-H. (2018) CTAB-PEG DNA Extraction from Fungi with High Contents of Polysaccharides. Molecular Biology, 52, 621-628.

ABSTRACT: Polymerase Chain Reaction (PCR) amplification of genomic DNA is a crucial step for molecular studies such as sequencing and genotyping because it requires pure and high-quality DNA. However, ...
Europe PMC

Improved genomic DNA extraction from citrus species using a modified CTAB method.

Citrus species are recalcitrant to DNA extraction due to high levels of polysaccharides, polyphenols, and secondary metabolites that interfere with DNA yield and purity. This study presents an ...
PNAS

Histone variant H2A.W7 represses meiotic crossover formation in Arabidopsis heterochromatin

Meiotic recombination is an evolutionary-conserved process that shapes genetic variation in most eukaryotes. DNA is packaged into nucleosomes, and high levels of nucleosome occupancy suppress ...
C&EN

An Improved Method and Device for Nucleic Acid Isolation Using a High-Salt Gel Electroelution Trap

Research Center for Translational Medicine, Sirius University of Science and Technology, Olympic Ave., 1, Sochi 354340, Russian Federation Andrey A. Zamyatnin Jr. Andrey A. Zamyatnin, Jr. Department ...
Pharmaceutical Technology

The role of pharma-grade Cetrimonium Bromide (CTAB) in downstream vaccine processing

Downstream vaccine processing aims to isolate the desired antigens and remove unwanted material through purification, precipitation and centrifugation. These steps are essential for the production of ...
Frontiers

A Combined RNA Preservation and Extraction Protocol for Gene Expression Studies in Cacao Beans

Despite the high economic importance of cacao beans, few RNA-based studies have been conducted on this plant material and hence no optimal RNA-extraction has been reported. Moreover, extraction of ...
Frontiers

DNA Extraction Method Plays a Significant Role When Defining Bacterial Community Composition in the Marine Invertebrate Echinometra mathaei

The microbial assemblages of marine organisms play fundamental biological roles in their eukaryotic hosts. Studies aimed at characterizing this diversity have increased over the last decade and with ...
Europe PMC

Effects of DNA extraction and purification methods on real-time quantitative PCR analysis of Roundup Ready soybean.

The quality of DNA affects the accuracy and repeatability of quantitative PCR results. Different DNA extraction and purification methods were compared for quantification of Roundup Ready (RR) soybean ...
Nature

A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide

We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from plant tissues. In contrast to the original method, the ...
C&EN

Comparison of Several Methods for the Extraction of DNA from Potatoes and Potato-Derived Products

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