Edwin Hauw and Tilmann Burckstummer discuss how automation and multimodal analysis are addressing long-standing limitations of optical pooled screening, enabling more scalable and informative CRISPR ...

CFG offers massively parallel genetic perturbations and genetic screening for unbiased discovery. Examples of our toolbox include genome-wide or custom CRISPR-based loss- and gain-of-function screens, ...

Lentiviral (LV) vectors have emerged as powerful tools for treating genetic and acquired human diseases. As clinical studies and commercial demands have progressed, there has been a growing need for ...

Article subjects are automatically applied from the ACS Subject Taxonomy and describe the scientific concepts and themes of the article. Despite highly successful applications of scRNA-seq, ...

In this work, we developed an imaging-based pooled-library CRISPR screening approach that provides readouts of both phenotype and genotype of individual cells by high-resolution, high-content imaging.

Genes are made up of DNA and carry the instructions necessary to build an organism. Humans have over 20,000 genes, while other animals, such as fruit flies, have about 14,000. An ongoing challenge in ...

CRISPR-based genetic screens are accelerating biological discovery, but current methods have inherent limitations. Widely used pooled screens are restricted to simple readouts including cell ...

CRISPRAnalyzeR is now available for download - see below or check out the wiki. You can use the provided CRISPRAnalyzeR web-service or download the suite for ...

The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/jacs.6b02921. Smart citations by scite.ai include citation statements extracted from the full ...

SQSTM1 is an adaptor protein that integrates multiple cellular signaling pathways and whose expression is tightly regulated at the transcriptional and post-translational level. Here, we describe a ...

The systematic discovery of new gene and drug combinations that modulate complex biological phenotypes and human diseases requires scalable and multiplexed screening technologies. We leverage the ...